Optical imaging through scattering media via magnetically modulated fluorescence.
نویسندگان
چکیده
A weak (< 1000 G) magnetic field can influence photochemical processes through its effect on electron spin dynamics in a photogenerated radical pair. In a solution of pyrene and dimethylaniline this effect manifests as magnetic field-dependent exciplex fluorescence. Here we describe magnetofluorescence imaging (MFI). A localized magnetic null defines a fluorescence detection volume, which is scanned through a sample to create an image. MFI forms an image without lenses and in the presence of arbitrarily strong optical scattering. The resolution of MFI is in principle not limited by optical diffraction, although the present implementation is far from the diffraction limit.
منابع مشابه
Magnetically modulated fluorescent probes in turbid media
Clemson University Department of Chemistry, 371 Hunter Laboratories, Clemson, SC. 29634 * Corresponding author: [email protected] Abstract Magnetically modulated optical nanoprobes (MagMOONs) were used to detect and distinguish probe fluorescence from autofluorescent backgrounds in turbid media. MagMOONs are micro/nano-sized particles with magnetically controlled orientation and orientation-de...
متن کاملEngineering of core/shell nanoparticles surface plasmon for increasing of light penetration depth in tissue (modeling and analysis)
Objectives: In this article, a new procedure for increasing the light penetration depth in a tissue is studied and simulated. It has been reported that the most important problem in biomedical optical imaging relates to the light penetration depth, and so this makes a dramatic restriction on its applications. In the optical imaging method, the detection of the backscattered pho...
متن کاملUltrasound-Modulated Fluorescent Contrast Agent for Optical Detection of Cancer Lesions in Deep Tissue
Optical imaging in a highly scattering medium is effective only at very shallow depths which limits its use as a diagnostic tool in biomedical imaging, particularly for tumor detection. By combining optical and acoustic modalities, high-contrast, tumor state-relevant optical information at higher spatial resolutions can be achieved. Hybrid imaging modalities such as acousto-optic and photoacous...
متن کاملFocused fluorescence excitation with time-reversed ultrasonically encoded light and imaging in thick scattering media.
Scattering dominates light propagation in biological tissue, and therefore restricts both resolution and penetration depth in optical imaging within thick tissue. As photons travel into the diffusive regime-typically 1 mm beneath human skin, their trajectories transition from ballistic to diffusive due to increased number of scattering events, which makes it impossible to focus, much less track...
متن کاملDetermination of optical properties of turbid media spanning visible and near-infrared regimes via spatially modulated quantitative spectroscopy.
We present a novel, noncontact method for the determination of quantitative optical properties of turbid media from 430 to 1050 nm. Through measuring the broadband reflectance from an unknown sample as a function of the spatial frequency of the projected illumination patterns, the absolute absorption and reduced scattering coefficients can be calculated without a priori assumptions of the chrom...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Optics express
دوره 18 25 شماره
صفحات -
تاریخ انتشار 2010